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. 2022 Jan 31;22:30. doi: 10.1186/s12906-022-03520-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Antioxidant activities (A) and DNA protection activity at 1mg/ml (B) of hydroponically (HyL) and soil grown (SoL) lettuce. Values expressed as means (n = 3) ± SD with *p < 0.05 in comparison with positive control representing “a”, SoL 1 mg/ml representing “b”, SoL 0.5 mg/ml representing “c” and SoL 0.25 mg/ml representing “d” with all respective concentrations of HyL and controls. [Lane# L = DNA ladder (1Kb), P = pBR322 plasmid, X = pBR322 plasmid treated with FeSO4 and H2O2 (control), 1 = pBR322 plasmid + 1 mg/ml of hydroponically grown lettuce; control for the pro-oxidant effect of the extract on DNA, 2 = plasmid + 1 mg/ml of hydroponically grown lettuce + FeSO4 + H2O2, 3 = pBR322 plasmid + 1 mg/ml of soil grown lettuce; control for the pro-oxidant effect on DNA, 4 = plasmid + 1 mg/ml of soil grown lettuce + FeSO4 + H2O2, 5 = plasmid + 1 mg/ml of Vitamin E + FeSO4 + H2O2]