Table 1.
Ultra-high performance liquid chromatography gradient parameters for our proposed method
| A) Parameters for the 22-minute runtime | B) Parameters for the 14-minute runtime | ||||||
|---|---|---|---|---|---|---|---|
| CS22-LFR and HS22-LFR | CS14-LFR | ||||||
| Time (min) | Flow (¼L/min)a | Solvent A (%)b | Solvent B (%)c | Time (min) | Flow (¼L/min)a | Solvent A (%)b | Solvent B (%)c |
| Initial | 50 | 60 | 40 | Initial | 50 | 60 | 40 |
| 2 | 57 | 43 | 1 | 57 | 43 | ||
| 12 | 46 | 54 | 6 | 46 | 54 | ||
| 12.10 | 30 | 70 | 6.10 | 30 | 70 | ||
| 18 | 1 | 99 | 9 | 1 | 99 | ||
| 20 | 1 | 99 | 12.00 | 1 | 99 | ||
| 20.10 | 60 | 40 | 12.10 | 60 | 40 | ||
| 22 | 60 | 40 | 14 | 60 | 40 | ||
a
Flow rate of the mobile phase.
b
Solvent composition: acetonitrile/water (60/40, v/v) mixture containing ammonium formate (10 mM, pH 6.2) and formic acid (0.1%).
c
Solvent composition: 2-propanol/acetonitrile (90/10, v/v) mixture containing ammonium formate (10 mM, pH 6.2) and formic acid (0.1%).