Table 3.
MTHFD1 genes in mothers and risk of congenital heart disease in offspring
| SNPs | Univariate logistic regression | Multivariable logistic regressionc | |||||
|---|---|---|---|---|---|---|---|
| cOR | 95% CI | P | aOR | 95% CI | P | FDR_Pd | |
| rs1950902 | |||||||
| G/G | 1.00 | – | 1.00 | – | – | ||
| G/A | 1.29 | 0.85–1.96 | 0.224 | 1.38 | 0.80–2.39 | 0.247 | 0.309 |
| A/A | 1.80 | 1.18–2.73 | 0.006 | 1.73 | 1.01–2.97 | 0.046 | 0.090 |
| Additivea | 1.36 | 1.12–1.64 | 0.002 | 1.30 | 1.02–1.65 | 0.025 | 0.090 |
| rs2236225 | |||||||
| G/G | 1.00 | – | 1.00 | – | – | ||
| G/A | 1.21 | 0.92–1.59 | 0.178 | 1.16 | 0.81–1.65 | 0.427 | 0.493 |
| A/A | 1.16 | 0.61–2.20 | 0.648 | 1.03 | 0.47–2.27 | 0.937 | 0.937 |
| Additive | 1.15 | 0.92–1.44 | 0.211 | 1.09 | 0.82–1.45 | 0.546 | 0.585 |
| rs2236222 | |||||||
| A/A | 1.00 | – | 1.00 | – | – | ||
| G/A | 1.27 | 0.97–1.66 | 0.087 | 1.27 | 0.96–1.67 | 0.096 | 0.144 |
| G/G | 2.50 | 1.46–4.29 | 0.001 | 2.38 | 1.38–4.12 | 0.002 | 0.015 |
| Additive | 1.42 | 1.16–1.75 | 0.001 | 1.40 | 1.14–1.73 | 0.002 | 0.015 |
| rs11849530 | |||||||
| A/A | 1.00 | – | 1.00 | – | – | ||
| G/A | 0.91 | 0.69–1.20 | 0.498 | 1.24 | 0.87–1.77 | 0.243 | 0.309 |
| G/G | 1.13 | 0.77–1.65 | 0.536 | 1.68 | 1.02–2.77 | 0.042 | 0.090 |
| Additive | 1.02 | 0.86–1.22 | 0.809 | 1.28 | 1.02–1.62 | 0.037 | 0.090 |
| rs1256142 | |||||||
| G/G | 1.00 | – | 1.00 | – | – | ||
| G/A | 1.44 | 1.03–2.02 | 0.035 | 1.57 | 1.01–2.45 | 0.048 | 0.090 |
| A/A | 1.20 | 0.83–1.74 | 0.340 | 1.57 | 0.97–2.56 | 0.068 | 0.113 |
| Dominantb | 0.92 | 0.70–1.21 | 0.550 | 1.57 | 1.03–2.40 | 0.037 | 0.090 |
Abbreviations: CI confidence interval; cOR crude odds ratio; aOR adjusted odds ratio; SNPs single nucleotide polymorphisms; MTHFD1 methylenetetrahydrofolate dehydrogenase 1; FDR_P false discovery rate P value
aAdditive means wild type homozygotes vs. heterozygote vs. mutant type homozygote
bDominant means wild type homozygote vs. mutant type homozygotes and heterozygote
cAdjusted for residence location, maternal education level (years), annual income in the past 1 year (RMB), history of adverse pregnancy outcomes, consanguineous marriage, history of congenital malformations in family, cold or fever in the periconceptional period, and personal lifestyle and habit in the periconceptional period including drinking alcohol, drinking tea, living near environmental pollution source, dyeing hair or perming and folate use
dFDR_P < 0.1 was considered to indicate a statistically significant difference