Skip to main content
. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Hippocampus. 2020 Oct 21;31(7):770–789. doi: 10.1002/hipo.23271

Figure 1. Absence of parvalbumin (PV+) cell bodies in midline thalamus.

Figure 1.

A: Representative coronal section (β −2.28mm) showing PV+ cell body expression throughout several regions of the brain. PV+ expression shown in red and DAPI in blue. Overlay shown adapted from Swanson (2018) to highlight thalamic structures.

B-E: Confocal images showing PV immunoreactivity in PVT (B), RE (C), TRN (D) and SSp (E). Neither PVT nor RE contain PV+ cell bodies, however PV immunoreacted puncta was abundant near or between their cell bodies (B-C insets). Scale bar = 300μm. Inset scale bar= 16μm.

D: PV+ cell bodies with a characteristic large immunonegative nuclei were seen in TRN (D inset).

E: PV+ cell bodies were also observed in SSp cortex showing their distinct sparse but organized layer distribution (E inset). Gold squares represent regions of 60X magnification shown in inset.

Abbreviations: β, bregma; CA1, CA1 subfield of the hippocampus; CA3, CA3 subfield of the hippocampus; DAPI, 4′,6-Diamidino-2-phenylindole dihydrochloride; DG, dentate gyrus; PV, parvalbumin; PVT, paraventricular; PRe, perireuniens; RE, nucleus reuniens; RH, rhomboid; SSp, primary somatosensory cortex; TRN, thalamic reticular nucleus.