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(a) Incubation of the 4IB-VHLGGQGYK peptide yields 19.6% deamidated products after 176 days. (b) Addition of ammonia greatly accelerates the deamidation process, yielding 9.4% deamidation within 14 days. (c,d) RDD confirms that the leading deamidation peak contains the canonical Glu residue, while the latter peak contains the isoGlu product.
