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Aging results for 4IB-VHLGGEGYK peptide under acidic conditions. (a) Only one peak is observed by LCMS, suggesting that no isoGlu has formed within 61 days. (b) Trace amounts of a coeluting species at −18 Dalton, consistent with the glutarimide, is observed during the trailing edge of the main peak. (c) RDD fragmentation confirms the presence of the glutarimide intermediate.
