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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: FASEB J. 2020 Oct 20;34(12):16536–16551. doi: 10.1096/fj.202001107R

Fig. 6. MiR-375 KO mutation and behavioral phenotypes.

Fig. 6.

(A) Comparison of circadian rhythm in miR-375 KO mutant (w1118; TI{GAL4}mir-375KO) and control (w1118). Data represent mean±SEM (n = 50–70). ns, nonsignificant; *, p<0.05; **, p<0.01; ***, p< 0.001 (one-way ANOVA test).

(B) Locomotor behavior under LD cycle and constant darkness. Representative double plotted actograms of w1118 and miR-375 KO mutant (w1118; TI{GAL4}mir-375KO) flies. White indicates the light phase; gray indicates the dark phase.

(C) Analysis of timeless levels in the Ago1-RNA complex in heads of miR-375 KO mutant and w1118. Error bar indicates SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (two-tailed t-test).

(D) Locomotor behavior under LD cycle and constant darkness. Representative double plotted actograms of w1118 and miR-375 KO mutant (w1118; TI{GAL4}mir-375KO) flies. White indicates the light phase; gray indicates the dark phase.

(E) Sleep analysis of male flies with overexpression of miR-375 driven by iso-tim-gal4. The colors of gray dark, light dark, and blue represent w1118 and miR-375 KO mutant (w1118; TI{GAL4}mir-375KO) flies sleep traces.

(F) Sleep profiles of male flies entrained under three LD cycles in miR-375 KO mutant (w1118; TI{GAL4}mir-375KO) and control (w1118). Data represent mean ± SEM. **, p<0.01; ***, p< 0.001 (Student’s t-test).

(G) Levels of timeless mRNA were altered in miR-375 KO (w1118; TI{GAL4}mir-375KO) flies. The relative expression levels were normalized to actin levels and were further normalized to control at ZT2. Error bar indicates SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.01 (two-tailed t-test).