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. 2021 Nov 19;9(2):2102678. doi: 10.1002/advs.202102678

Figure 18.

Figure 18

Uptake of MBG nanoparticles by bone marrow‐derived dendritic cells (BMDCs). A) Flow cytometry analysis of unstimulated (green, US), or LPS (red) or Poly I:C (PI:C, blue) stimulated BMDCs treated or untreated (gray) with fluorescein isothiocyanate (FITC)‐labeled‐MBG nanoparticles during 24 h. Graphs show the percentage of FITC+ cells (middle panel) or the median of fluorescence intensity (MFI, right panel). Immature as well as mature BMDCs take up the nanosphere. B) Confocal microscopy analysis of BMDCs after incubation of 2 h with FITC‐MBG nanoparticles shows the cytosolic distribution of nanospheres. FITC‐MBG nanoparticles are shown in green, in blues is shown cell nucleus stained with Hoescht dye. C) Unstimulated, or LPS‐ or Poly I:C‐stimulated BMDCs were incubated (gray bars) or not (light blue bars) with nanospheres during 24 h and evaluated for the expression of CD11c, CD40, CD86, MHC II, CD80 surface markers. Graphs show the MFI. MBG nanoparticles do not alter the maturation status of BMDCs, except the expression of CD86. D) ELISA assay for the detection of IL‐6 in 24 h culture supernatants of BMDCs incubated (gray bars) or not (light blue bars) with nanospheres, in the presence or without stimuli. MBG nanoparticles do not induce the proinflammatory IL‐6 cytokine in immature BMDCs (US), indicating that these nanomaterials do not induce DC maturation by themselves. Reproduced under the terms of CC‐BY license open access.[ 229 ] Copyright 2020, MDPI.