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. 2022 Jan 18;12:814465. doi: 10.3389/fpls.2021.814465

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Copyright © 2022 Sun, Song, Chang, Wang, Lu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Map-based cloning of OsLMP1. (A) The OsLMP1 locus was mapped to a 3-cM interval on chromosome 9L between indel markers Indel1-1 and Indel1-2. (B) The location of the OsLMP1 locus was narrowed down to a 110-kb region between indel markers 1,715 and 1,823 in the BAC clone OSJNBa0010B06 using 950 F₂ homozygous mutant plants. (C) Eight open reading frames (ORFs) were predicted in the mapped region. Structure of OsLMP1. ATG, TAA: start and stop codons, respectively. Boxes: exons. Lines between black boxes: introns. One single nucleotide insertion results in a frame shift mutation in lmp1-1. The deletion site in crisp-m1 is labeled. The black box denotes the coding sequence from ATG to TAA. (D,E) Comparison of the mutant site of LOC_Os09g32740 between the wild-type (D) and lmp1-1 mutant (E). The inserted base is labeled in red in (E).