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. 2022 Jan 18;12:814465. doi: 10.3389/fpls.2021.814465

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Copyright © 2022 Sun, Song, Chang, Wang, Lu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular location and the expression pattern of OsLMP1. (A–D) The 35S:OsLMP1:GFP vector was transformed into rice protoplasts, and the green fluorescent signal was visualized using confocal microscopy. OsMADS3, which localizes to the nuclei, was selected as the marker. The results revealed colocalization of the GFP signal with OsMADS3 fluorescence, indicating that OsLMP1 is mainly targeted to the rice nucleus. Bar: 100 μm. (E–H) Transgenic plants containing the OsLMP1-1391Z vector were stained using GUS dye at the flowering stage. (E) Stem; (F) leaf blade; (G) root; (H) panicle. The results showed that the stem, leaf blade and root were more deeply stained, whereas the panicle was weakly stained. (I) qRT-PCR analysis of OsLMP1 in the root, seedling, flag leaf, stem, sheath, and panicle tissues of the wt. The results showed that OsLMP1 is mainly expressed in young leaf at the early tiller stage.