Figure 3.

In vivo intraoperative LN tracing and ex vivo LN Raman imaging 10 min after injection of GERTs. A) Real‐time LNs tracing with a hand‐held Raman scanner (785 nm, 5.0 × 10³ W cm⁻² power density, 0.2 s acquisition time). Raman signals were collected on the injection site (IS), LNs, and fat. B) Photograph of a rabbit after GERTs injection and skin removal. The black circle indicates the IS. C) Enlarged area (18 ×) indicated in panel (B). The white circles indicate the SLN, 2^nd LN, and the fat tissues. Raman spectra D) from different positions and E) five representative in situ scanning points (1: top, 2: left, 3: bottom, 4: right, 5: center) on the SLN. F–H) Photograph, Raman image, and the overlay of a dissected SLN, 2^nd LN, and adjacent fat tissue. Raman image was plotted using the Raman band at 1340 cm⁻¹ (shaded area in (D)). I) Characteristic Raman signal of the position which marked by white arrows (in panel (H)) on the dissected SLN, 2^nd LN, and fat. J) H&E‐stained micrograph of a SLN section after GERTs injection. K,L) Bio‐TEM images of GERTs distributed in the organelle of lymphocyte. The scale bars are F–H) 1 mm, J) 500 µm, and L) 100 nm.