Table 1.
Passive and active membrane properties of prefrontal and hippocampal neurons from neonatal and prejuvenile CON and GE mice in vitro
| Membrane Properties | Layer 5/6 |
Layer 2/3 |
HP |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CON | GE | p | CON | GE | p | CON | GE | p | |||
| Neonatal (P8–P10) | Passive | Rin (MΩ) | 401.8 ± 19.5 | 427.6 ± 35.1 | 0.5352 | 471.3 ± 18 | 536.1 ± 35.8 | 0.0699 | 453.8 ± 19.8 | 361.3 ± 16.1 | 0.0008 |
| Cm (pF) | 125.1 ± 5.6 | 125.6 ± 13.9 | 0.9693 | 94.2 ± 4.4 | 90.5 ± 3.3 | 0.5804 | 118.1 ± 5.8 | 109.7 ± 5.52 | 0.2559 | ||
| τm (ms) | 83.9 ± 4.3 | 81.4 ± 8.0 | 0.7876 | 67.2 ± 3.5 | 73.4 ± 4.6 | 0.2879 | 49.6 ± 2.6 | 41.4 ± 2.9 | 0.0376 | ||
| RMP (mV) | −68.4 ± 0.7 | −67.2 ± 0.9 | 0.3986 | −69.8 ± 0.9 | −65.8 ± 1.1 | 0.0096 | −69.2 ± 0.8 | −69.0 ± 0.7 | 0.3016 | ||
| Sag (%) | 10.2 ± 0.9 | 12.8 ± 2.5 | 0.2225 | 4.4 ± 0.62 | 4.58 ± 0.38 | 0.8457 | 23.7 ± 1.2 | 29.3 ± 1.2 | 0.0028 | ||
| Active | AP threshold (mV) | −42.4 ± 1.3 | −42.8 ± 2.1 | 0.9080 | −38.8 ± 1.2 | −39.1 ± 1.3 | 0.8544 | −40.5 ± 1.0 | −43.0 ± 1.3 | 0.1148 | |
| AP amplitude (mV) | 71.5 ± 1.3 | 69.4 ± 2.2 | 0.8525 | 66.8 ± 1.4 | 59.5 ± 1.8 | 0.0028 | 80.7 ± 1.4 | 81.9 ± 1.4 | 0.5591 | ||
| AP halfwidth (ms) | 3.56 ± 0.15 | 3.40 ± 0.18 | 0.5970 | 2.85 ± 0.06 | 3.38 ± 0.15 | 0.00062 | 2.05 ± 0.04 | 2.25 ± 0.07 | 0.0085 | ||
| Rheobase (pA) | 43.5 ± 3.6 | 40.4 ± 45.0 | 0.6716 | 43.1 ± 2.9 | 46.6 ± 6.8 | 0.5710 | 58.3 ± 2.7 | 59.3 ± 2.6 | 0.7863 | ||
| Firing rate (Hz) | 12.3 ± 0.7 | 11.3 ± 1.1 | 0.4956 | 12.5 ± 1.0 | 11.3 ± 1.6 | 0.4945 | 12.5 ± 0.7 | 12.3 ± 0.8 | 0.7438 | ||
| Prejuvenile (P20–P24) | Passive | Rin (MΩ) | 222.2 ± 11.5 | 188.9 ± 17.9 | 0.1146 | 235.4 ± 13.2 | 237.8 ± 9.7 | 0.8938 | 239.0 ± 19.6 | 211.9 ± 24.7 | 0.3703 |
| Cm (pF) | 178.2 ± 12.5 | 184.7 ± 13.9 | 0.7547 | 131.3 ± 9.37 | 115.2 ± 8.4 | 0.2409 | 174.9 ± 24.0 | 131.9 ± 22.8 | 0.1997 | ||
| τm (ms) | 59.6 ± 5.8 | 45.9 ± 4.7 | 0.1353 | 46.9 ± 3.64 | 44.5 ± 2.9 | 0.6337 | 37.7 ± 1.6 | 33.2 ± 5.1 | 0.3286 | ||
| RMP (mV) | −69.5 ± 0.5 | −69.3 ± 0.5 | 0.8084 | −66.9 ± 0.5 | −69.7 ± 0.4 | 0.00076 | −64.9 ± 1.1 | −63.5 ± 2.0 | 0.5000 | ||
| Sag (%) | 16.2 ± 1.7 | 18.4 ± 2.7 | 0.4839 | 6.4 ± 1.6 | 4.3 ± 0.7 | 0.3337 | 27.3 ± 2.0 | 21.1 ± 2.8 | 0.0664 | ||
| Active | AP threshold (mV) | −46.1 ± 1.3 | −48.4 ± 2.0 | 0.3297 | −44.5 ± 1.2 | −46.4 ± 1.7 | 0.3370 | −45.4 ± 1.8 | −49.3 ± 1.9 | 0.1325 | |
| AP amplitude (mV) | 90.1 ± 1.1 | 90.9 ± 1.6 | 0.6539 | 88.5 ± 1.1 | 86.8 ± 1.3 | 0.3388 | 97.1 ± 2.7 | 96.8 ± 4.4 | 0.9528 | ||
| AP halfwidth (ms) | 1.67 ± 0.05 | 1.53 ± 0.07 | 0.6839 | 1.79 ± 0.06 | 1.65 ± 0.07 | 0.1186 | 1.73 ± 0.04 | 1.74 ± 0.06 | 0.8715 | ||
| Rheobase (pA) | 73.8 ± 5.2 | 78.6 ± 9.3 | 0.6231 | 85.1 ± 6.2 | 89.5 ± 7.8 | 0.6694 | 50.7 ± 2.7 | 48.4 ± 5.1 | 0.6444 | ||
| Firing rate (Hz) | 11.4 ± 0.8 | 10.2 ± 1.3 | 0.4260 | 11.8 ± 0.7 | 10.6 ± 1.1 | 0.3109 | 15.0 ± 1.2 | 15.9 ± 1.8 | 0.7774 | ||
Data are shown as mean ± SEM. Significance was assessed using one-way ANOVA test followed by Bonferroni-corrected post hoc test, and the listed p values correspond to comparisons between CON and GE mice for the neurons in the same region.