Figure 4.

Changes in response patterns of individual neurons. a, Color maps show the averaged Ca²⁺ responses to 12 moving-bar stimuli of 281 neurons recorded from an example imaging site. Each line indicates an individual neuronal response during awake (left) and isoflurane (right) conditions. The order of the neurons (“Neuron #”) is the same in both panels. DS cells and weak DS cells are separated by dotted lines. The preferred direction of each DS and weak DS cell is labeled with red and pink circles, respectively. b, OS responses are illustrated as in a for the DS responses. The preferred orientation of each OS and weak OS cell are labeled with blue and cyan circles, respectively. The amplitude of ΔF/F₀ in a and b is color coded according to the color bar on the right in b. c, Summary of the changes in six response types (DS, OS, weak DS, weak OS, nonselective, and no response) from awake to anesthetic condition. d, Changes in the DSI of individual DS or weak DS cells, shown by plotting the indices in the awake condition on the horizontal axis and those in the isoflurane condition on the vertical axis. Each dot indicates an individual neuron. Colors of dots indicate the individual imaging sites (n = 35). The value of the correlation coefficient (R) is inserted in each panel. e, Change in OSI of individual OS or weak OS cells as shown in d.