Figure 1.

Genotyping accuracy in synthetic polyploids. (A) Sensitivity and precision by depth for each caller on real diploid, as well as synthetic tetraploid and hexaploid Illumina data sets. (B) Counts of false-positive biallelic calls stratified by depth and genotype (top). False-negative rates at biallelic sites stratified by depth and genotype (bottom). (C) Precision-recall curves for a various tetraploid sequencing depths. Score metrics used to generate the curves were RFGQ (Octopus), GQ (GATK4), and GQ (FreeBayes).