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. 2022 Jan 18;12:783806. doi: 10.3389/fimmu.2021.783806

Figure 7.

Figure 7

Microbiota transplantation from melatonin-treated mice alleviates oxazolone (Oxa)-induced colitis. (A) The process of fecal microbiota transplantation. The mice were treated with antibiotics for 7 days to clear the gut microbiota (day 1 to day 7) and transplanted feces from 7-day melatonin-treated or melatonin-untreated mice (day 8 to day 14) before 3% oxazolone enema (day 15). Mice were sacrificed on day 16. (B) Body weight changes in fecal microbiota transplantation (FMT) mice at day 16. (C, D) Colon length in FMT mice at day 16. (E–G) Representative H&E staining (×20) and pathological score of the inflamed gut epithelium. (H, I) qPCR of Tnfa and Il-1b mRNA in colonic tissues in FMT mice at day 16 after 3% oxazolone enema (all values were normalized to MT0 group). (J–L) Immunoblot and densitometric analyses of ZO-1 and occludin in FMT mice at day 16 after 3% oxazolone enema. (M, N) qPCR of Il-5 and Il-13 mRNA in colonic tissues in FMT mice at day 16 after 3% oxazolone enema (all values were normalized to MT0 group). Data are representative of two independent experiments. Data are shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001. ns, no significance.