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. 2021 Dec 30;13(1):1025–1038. doi: 10.1080/21655979.2021.2017566

Figure 2.

Figure 2.

miR-128-3p mainly affects CD4+ CD25+ Foxp3+ Treg subset. (a) Flow chart of lymphocyte isolation and coculture experiment. (b, c) The upregulation and downregulation of miR-128-3p expression was detected by RT–qPCR. After 48 hours of the coculture of T lymphocytes and GES-1 cells infected with control or LV-miR-128-3p-inhibitor or the supernatant of treated cells, the populations of CD4+ CD25+ Foxp3+ Tregs (d, e) and CD8+ CD28-CD25+ Foxp3+ Tregs (h, i) were determined by flow cytometry. (f, g) HGC-27 cells transfected with the miR-128-3p mimic or control or the supernatant of these treated cells were cocultured with T lymphocytes for 48 h, and then, the populations of CD4+ CD25+ Foxp3+ Tregs were determined by flow cytometry. *P < 0.05, **P < 0.01.