Figure 3.

B7-H4 positively regulates the proliferation, migration and invasion of CRC cells. a, b. The expression levels of B7-H4 protein and mRNA in normal human colonic epithelial cells (FHC) and CRC cells (HT29, HCT116, HCT8, LOVO, SW420 and SW620) were detected by Western blot (a) and RT-qPCR (b), respectively. c. SW620 and LOVO cells were transfected with si-B7-H4, B7-H4 expression plasmid or the corresponding control. B7-H4 expression level in each group was detected by Western blot. d. The proliferation ability of CRC cells was examined by CCK-8. e and f. The migration (e) and invasion (f) ability of CRC cells was evaluated by transwell assay. *, P < 0.05, **, P < 0.01, and ***, P < 0.001. The error bars are defined as s.d