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. 2021 Dec 29;13(1):395–406. doi: 10.1080/21655979.2021.2005929

Figure 4.

Figure 4.

KLF9 transcription inhibits the expression of DDAH2. (a) The binding sites between KLF9 and DDAH2 promoter was predicted by JASPAR. (b) The luciferase activity of the DDAH2 detected by dual luciferase reporter assay. ***p < 0.001 vs sh-NC. (c) The combination between KLF9 and DDAH2 promoter detected by ChIP. ***p < 0.001 vs IgG. The (d) protein and (e) mRNA levels of DDAH2 in HG-induced HTR8/SVneo cells. **, ***p < 0.01, 0.001 vs control. The (f) protein and (g) mRNA levels of DDAH2 in HG-induced HTR8/SVneo cells transfected with sh-KLF9. ***p < 0.001 vs sh-NC.