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. 2021 Dec 29;13(1):395–406. doi: 10.1080/21655979.2021.2005929

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — DDAH2 is involved in the inhibition of sh-KLF9 on the cell viability of HG-induced HTR8/SVneo cells. The (a) protein expression and (b) mRNA level of DDAH2 after transfection with sh-NC, sh-DDAH2#1, sh-DDAH2#1 was detected. ***p < 0.001 vs sh-NC. HTR8/SVneo cells were induced with HG and transfected with sh-NC/sh-KLF9 or co-transfected with sh-KLF9 and sh-NC/sh-DDAH2. (c)The cell viability was detected by CCK-8 assay. (d) Cell apoptosis was determined using TUNEL assay. (e) apoptosis-associated protein levels were measured by Western blot. ***p < 0.001 vs HG; ###p < 0.001 vs HG+sh-NC; +, ++, +++p < 0.05, 0.01, 0.001 vs HG+sh-KLF9+ sh-NC.