Figure 6.

The influence of miR-136-5p and YTHDF1 on CRC progression and chemoresistance. (a) YTHDF1 protein level was examined in HT-29 and LoVo cells transfected with vector or oe-YTHDF1. (b-o) HT-29 and LoVo cells were transfected with miR-136-5p mimic and oe-YTHDF1. Cell proliferation (b and c), colony formation (d and e), migration (f and g), invasion (h and i), 5-FU resistance (j and k), oxaliplatin resistance (l and m), β-catenin and cyclin D1 protein expression (n and o) were examined by CCK-8 assay, colony formation assay, transwell assay and WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001.