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. 2022 Jan 3;13(1):96–106. doi: 10.1080/21655979.2021.2008641

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — MYL9 affected cell proliferation, migration and invasion of colorectal cancer cells after overexpression or knockdown of MYL9. (a) Detection of MYL9 mRNA expression after transfection with siRNA-MYL9-1 or siRNA-MYL9-2 in HCT116 cells by RT-qPCR assay. ***P < 0.001 vs. siRNA-NC. (b) Inspection of overexpression of MYL9 in colorectal cancer cell lines was carried out by RT-qPCR. ***P < 0.001 vs. Ov-NC. (c) CCK-8 was adopted to detect cell proliferation levels. (d-e) Wound healing was used to evaluate the ability of cell migration. (f-g) Transwell assay was performed for cell invasion. (h). Western blot assay was used to test the expression of MMP2 and MMP9. **P < 0.01, ***P < 0.001 vs. siRNA-NC; ^###P < 0.001 vs. Ov-NC.