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. 2022 Jan 3;13(1):974–984. doi: 10.1080/21655979.2021.2016038

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — MiR-518a was a downstream gene of HOXA11-AS. (a) StarBase prediction of HOXA11-AS as a binding target of miR-518a. (b) Luciferase reporter assay showed luciferase activity in TU686/CDDP and TU177/CDDP cells transfected with NC mimics and miR-518a mimics. (c and d) RT-qPCR showed the relative miR-518a expression in CDDP-resistant tissues and cells. (e) RT-qPCR showed the relative miR-518a expression in TU686/CDDP and TU177/CDDP cells transfected with shNC, shHOXA11-AS#1, pcDNA3.1, or pcDNA3.1/ HOXA11-AS. (f) Pearson’s correlation analysis was used to assess the correlation between HOXA11-AS and miR-518a expression. *p < 0.05 and **p < 0.01.