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. 2022 Jan 3;13(1):974–984. doi: 10.1080/21655979.2021.2016038

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — SPATS2L was directly targeted by miR-518a. (a) StarBase website predicted 10 potential targets of miR-518a. (b) RT-qPCR revealed that relative SPATS2L expression in CDDP-resistant LSCC cells transfected with NC mimics or miR-518a mimics. (c) The binding sequences between miR-518a and SPATS2L were presented. (d) Luciferase reporter assay revealed the luciferase activities of SPATS2L-wt or SPATS2L-mut reporters in TU686/CDDP and TU177/CDDP cells transfected with NC mimics or miR-518a mimics. (e) RT-qPCR assay showed the expression of SPATS2L in in CDDP-resistant LSCC tissues (n = 27) compared with CDDP-sensitive LSCC tissues (n = 33). (f and g) RT-qPCR and Western blot assays showed the mRNA and protein level of SPATS2L in TU686/CDDP and TU177/CDDP cells. (h) Pearson’s correlation analysis showed the correlation between miR-518a and SPATS2L expression in CDDP-resistant LSCC tissues. *p < 0.05 and **p < 0.01.