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. 2022 Jan 3;13(1):1399–1410. doi: 10.1080/21655979.2021.2017700

Figure 5.

Figure 5.

Inhibitory effects of DEZ on inflammation and oxidative stress of IL-1β induced HNPCs were restored by MAPK pathway agonist PMA. (a) The expression of IL-6 and TNF-α was assessed by RT-qPCR. (b) ELISA was utilized to evaluate the contents of IL-6 and TNF-α. (c) The protein levels of p-NF-κB p65, p65 expression in nucleus, p65 expression in cytoplasm and cox-2 was examined with Western blot analysis. (d) The ROS content was tested by means of the 2ʹ, 7ʹ-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method. The levels of (e) MDA and (f) GSH were measured using commercial kits. (g) Western blot assay was utilized to determine the protein levels of SOD1 and SOD2. ***P < 0.001 vs. untreated group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. IL-1β group; ΔP<0.05, ΔΔP<0.01, ΔΔΔP<0.001 vs. IL-1β+DEZ group.