Figure 3.

Effect of LAP on DOX-induced oxidative stress in H9c2 cells. H9c2 cells were treated with 1 μM DOXwith or without 1–3 μM LAP for 24 h. A, ROS level was detected by DCFH-DA staining. Original magnification: x200. The activity of SOD (b), CAT (c) and GSH (d) was assessed in DOX-induced cells with or without different doses of LAP. LAP: Lapatinib; DOX: Doxorubicin; ROS: Reactive oxygen species; SOD: superoxide dismutase; CAT: Catalase; GSH: Glutathione. Data are expressed as mean ± SD calculated from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus DOX