TABLE 1.
Survival rate of H. pylori strain ATCC 43504T added to saliva after a short exposure to urea and HCl
| HCl concn (N)b | Survival (%) ata:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Urea concentration (mM)b
|
pH | |||||||||||
| 1,000 | 500 | 200 | 160 | 80 | 40 | 20 | 10 | 5 | 2.5 | 1.25 | ||
| 0.24 | 4.75 | 3.2 | 1.6 | 1.15 | 0.8 | 0.48 | 0.48 | 0.21 | 0.48 | 0.32 | 0.16 | 0.68 |
| 0.12 | 2.5 | 5.5 | 4.75 | 3.8 | 2.75 | 1.25 | 2.05 | 0.55 | 0.23 | 0.8 | 0.03 | 1.06 |
| 0.06 | 2.15 | 3.45 | 3.73 | 4.5 | 4.2 | 3.9 | 4.75 | 1.45 | 0.9 | 1.75 | 0.02 | 1.38 |
| 0.03 | 0.65 | 1 | 1.25 | 1 | 1.95 | 1.5 | 2.6 | 0.8 | 0.34 | 0.38 | 0.01 | 1.82 |
a
H. pylori survival rates are expressed as the percentage of input colonies recovered after urea-HCl treatment, as determined by parallel controls with PBS replacing urea and HCl. All values are means for triplicate trials.
b
Final concentration during incubation of H. pylori suspension with urea and HCl solution before dilution with PBS buffer.