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. 2021 Dec 24;13(1):155–163. doi: 10.1080/21655979.2021.2011634

Figure 3.

Figure 3.

Up-regulation of DHCR24 inhibited H2O2-induced oxidative stress and apoptosis of A549 cells. (a) Western blot analysis of DHCR24 (‘*p < 0.05 vs. +vector, n = 3). (b) The levels of SOD in A549 cells were detected by SOD activity assay (‘*’ p < 0.05 vs. +vector, n = 3). (c) The contents of ROS in A549 cells were detected by DHR-ROS test kit (‘*p < 0.05 vs. +vector, n = 3). (d) The Caspase-3 activity of A549 cells was detected (‘*p < 0.05 vs. +vector, n = 3). (e) Western blot analysis of Bcl-2 and Bax (‘*p < 0.05 vs. +vector, n = 3). (f) Results of TUNEL staining of A549 cells (200×) (‘*p < 0.05 vs. +vector, n = 3)