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. 2022 Jan 14;13(1):1908–1920. doi: 10.1080/21655979.2021.2020396

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Overexpressed CASP5 alleviates the effects of agomiR-527.

(a) CASP5 expression levels were detected by qRT-PCR. (b) Cell viability evaluated by CCK-8 assay. (c–e) Concentration of LDH, IL-1β, and IL-18. (f) Flow cytometry assay analyses of pyroptosis of HGFs. (g) Pyroptosis-related proteins analyzed by Western blotting assay for caspase-1, caspase-4, and GSDMD-N. **P < 0.01, vs. len-nc and control groups. ^#P < 0.05, ^##P < 0.01, vs. LPS+len-nc and LPS+agomiR-527+ len-nc group. ^&&P < 0.01, vs. LPS+agomiR-527+ len-nc group.