Figure 4.

LncRNA SNHG12 carried by CAFs-EVs into NSCLC cells and promoted DDP resistance in NSCLC cells. oe-SNHG12 was transfected in the cells to increase the expression of SNHG12 (oe-NC was used as the negative control). A: qRT-PCR was used to verify the transfection efficiency of SNHG12 and detect the expression of SNHG12 in CAFs-EVs; B: qRT-PCR was adopted to detect the expression of SNHG12 in CAFs-EVs; C: qRT-PCR was performed to detect the expression of SNHG12 in NSCLC cells after CAFs-EVs overexpressing SNHG12 (using CAFs-oe-EVs as the control) were co-cultured with NSCLC cells; D: CCK-8 was used to test the activity of NSCLC cells and the half inhibitory concentration (IC₅₀) of DDP; E: Colony formation assay was conducted to verify the proliferation of NSCLC cells; F: Flow cytometry was performed to detect the apoptosis level of NSCLC cells. The cell experiment was repeated 3 times independently, and data were expressed as mean ± standard deviation. Data in panels A-B and D were analyzed using one-way ANOVA and data in panels C-F were analyzed using two-way ANOVA, followed by Tukey’s post-hoc test, * p < 0.05.