Figure 5.

LncRNA SNHG12 promoted the RNA stability and the transcription of XIAP by binding to HuR. A: The binding of SNHG12 to HuR (ELAVL1), and HuR to XIAP were predicted by the database (http://www.rna-society.org/rnainter/); B-C: RNA pull-down and RIP (RNA immunoprecipitation) were conducted to verify the binding of SNHG12 to HuR in NSCLC cells; D- E: RNA pull-down and RIP were adopted to verify the binding of HuR to XIAP in NSCLC cells; si-HuR (si-HuR-1 and si-HuR-2) was transfected into NSCLC cells to down-regulate the expression of HuR, and si-NC was as the negative control; F: Western blot was conducted to test the transfection efficiency of si-HuR; G: Actinomycin D was used to test the half-life period of XIAP; H: qRT-PCR was performed to detect the level of XIAP mRNA. The cell experiment was repeated 3 times independently, and data were expressed as mean ± standard deviation. Data in panels B-E and G-H were analyzed using two-way ANOVA and data in panel F were analyzed using one-way ANOVA, followed by Tukey’s post-hoc test, * p < 0.05.