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. 2021 Dec 29;13(1):735–745. doi: 10.1080/21655979.2021.2012627

Figure 5.

Figure 5.

CAR inhibited the inflammation, oxidative stress and apoptosis in high-glucose-induced ARPE-19 cells by activating Nrf2/ARE signaling pathway. (a). After adding Nrf2 inhibitor ML385, the levels of inflammatory factors in cells were detected by ELISA kit. (b). Detection of oxidative stress levels was conducted in cells using corresponding kits. (C&D). TUNEL assay was utilized for apoptosis levels. (e). Detection of apoptosis-related protein expression levels Bcl-2, Bax, cleaved-caspase3 and cleaved-caspase9 in cells induced by HG was tested by Western blotting after CAR treatment. Data are expressed as mean ± SD. ***P < 0.001 versus control. ##P < 0.01, ###P < 0.001 versus HG. &P < 0.05, &&P < 0.01, &&&P < 0.001 versus HG+CAR.