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. 2022 Jan 5;13(1):1346–1358. doi: 10.1080/21655979.2021.2017565

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — MKLN1-AS downregulation impeded the malignant progression in HCC cells. (a) The knockdown effects of sh-MKLN1-AS#1, sh-MKLN1-AS#2, sh-MKLN1-AS#3 on the MKLN1-AS level were assessed by RT-qPCR. (b-d) The detection of cell proliferation was performed through CCK-8 (b), colony formation assay (c) and EDU assay (d) in HuH7 and LM3 cells transfected with sh-NC or sh-MKLN1-AS#1. (e) The examination of angiogenesis was performed by tube formation assay. (f-g) The determination of cell migration (f) and invasion (g) was performed by transwell assay. (h) The protein analysis of PCNA, cleaved-caspase 3, E-cadherin and N-cadherin was performed using Western blot. *P < 0.05, **P < 0.01, ***P < 0.001.