Figure 5.
Inhibition of miR-22-3p attenuated the function of MKLN1-AS knockdown in HCC cells. HuH7 and LM3 cells were transfected with sh-NC+anti-NC, sh-MKLN1-AS#1+ anti-NC or sh-MKLN1-AS#1+ anti-miR-22-3p. (a) RT-qPCR was applied to determine the miR-22-3p level. (b-d) CCK-8 assay (b), colony formation assay (c) and EDU assay (d) were applied to detect cell proliferation. (e) Tube formation assay was used to analyze the angiogenetic ability. (f-g) Transwell assay was used to examine cell migration (f) and invasion (g). (h) Western blot was used to measure the protein levels of PCNA, cleaved-caspase 3, E-cadherin and N-cadherin. **P < 0.01, ***P < 0.001.