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. 2022 Jan 5;13(1):1346–1358. doi: 10.1080/21655979.2021.2017565

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — ETS1 overexpression abrogated the anti-tumor function of miR-22-3p in HCC cells. Transfection of miR-NC+vector, miR-22-3p+vector and miR-22-3p+ETS1 was conducted in HuH7 and LM3 cells. (a) The protein expression of ETS1 was determined via Western blot. (b-d) Cell proliferation was evaluated via CCK-8 assay (b), colony formation assay (c) and EDU assay (d). (e) Angiogenesis was assessed via tube formation assay. (f-g) Cell migration (f) and invasion (g) were examined via transwell assay. (h) PCNA, cleaved-caspase 3, E-cadherin and N-cadherin protein levels were analyzed using Western blot. **P < 0.01, ***P < 0.001.