Figure 4.

DHA inhibits IL-17A-stimulated HaCaT cell over-proliferation by targeting FGFR1. The mRNA (a) and protein (b) expression of FGFR1 under the intervention of IL-17A and DHA were detected by RT-qPCR and Western blotting, respectively; (c) Cell viability following addition of FGFR1 agonist SUN11602 was detected by CCK-8; (d) Cell proliferation was detected with EDU staining; (e) Cell proliferation-related protein expression including Ki-67, cyclin D1 and c-Myc were analyzed by Western blotting. ***P < 0.001 vs. control. ##P < 0.01 and ###P < 0.001 vs. IL-17A. ^ΔP<0.05, ^ΔΔP<0.01 and ^ΔΔΔP<0.001 vs. IL-17A+DHA. DHA, dihydroartemisinin; FGFR1, fibroblast growth factor receptor 1.