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. 2022 Jan 5;13(1):1554–1564. doi: 10.1080/21655979.2021.2013028

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — miR-369-3p is a target of MSC-AS1 in OA chondrocytes.

(a) StarBase website predicted the binding site between MSC-AS1 and miR-369-3p. (b) Dual-luciferase reporter assay was performed to analyze the interaction between MSC-AS1 and miR-369-3p in OA chondrocytes. (c) RIP assay showed the enrichment of MSC-AS1 and miR-369-3p in OA chondrocytes of anti-Ago2 group compared with anti-IgG group. (d) RT-qPCR showed the relative expression of miR-369-3p in OA chondrocytes transfected with shMSC-AS1. (e) RT-qPCR showed the relative expression of miR-369-3p in OA patients compared with normal patients. (f) Pearson’s correlation analysis showed the correlation between MSC-AS1 and miR-369-3p expression in OA cartilage tissues. *p < 0.05.