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. 2021 Dec 30;13(1):440–454. doi: 10.1080/21655979.2021.2009317

Figure 2.

Figure 2.

Circ_0005320 knockdown suppresses OSCC cell growth, migration, invasion and induces cell apoptosis. (a-n) CAL27 and SCC25 cells were transfected with si-circ_0005320 or si-NC. (a, b) Detection of circ_0005320 expression in CAL27 and SCC25 cells using qRT-PCR. (c, d) CCK-8 assay for cell proliferation. (e) Colony formation assay for the colony formation rates of cells. (f, g) Transwell assay for cell migration and invasion. (h) Tube formation assay for cell tube formation ability. (i, j) Flow cytometry for the analysis of cell cycle and apoptosis. (k-n) Western blot analysis of PCNA, Bcl-2, Bax, cleaved caspase 3, and total caspase 3 protein levels in cells. *P < 0.05.