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. 2021 Dec 30;13(1):440–454. doi: 10.1080/21655979.2021.2009317

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Circ_0005320 knockdown suppresses OSCC cell malignant phenotypes by regulating miR-637. (a) qRT-PCR analysis of miR-637 expression in CAL27 and SCC25 cells transfected with anti-miR-637 or anti-miR-con. (b-p) CAL27 and SCC25 cells were transfected with si-NC, si-circ_0005320, si-circ_0005320 + anti-miR-con, or si-circ_0005320 + anti-miR-637. (b, c) qRT-PCR analysis of miR-486-3p expression in CAL27 and SCC25 cells. (d, e) CCK-8 assay for cell proliferation. (f) Colony formation assay for the colony formation rates of cells. (g, h) Transwell assay for cell migration and invasion. (i) Tube formation assay for cell tube formation ability. (j-l) Flow cytometry for the analysis of cell cycle and apoptosis. (m-p) Western blot analysis of PCNA, Bcl-2, Bax, cleaved caspase 3, and total caspase 3 protein levels in cells. *P < 0.05.