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. 2022 Jan 4;13(1):1411–1423. doi: 10.1080/21655979.2021.2018096

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Stability and cellular localization of circZNF652 in GBM cells. (a) and (b) The mRNA expression of circZNF652 and linear ZNF652 was examined using RT-qPCR after U172 and U87 cells treatment with RNase R. (c) and (d) The mRNA expression of circZNF652 and linear ZNF652 was examined using RT-qPCR after U172 and U87 cells treatment with actinomycin D. (e) and (f) The mRNA expression of U6, GAPDH, circZNF652 of nucleus and cytoplasm in U172 and U87 cells was detected using RT-qPCR. (g)-(k) The protein levels of lamin B and α-tublin of nucleus and cytoplasm in U172 and U87 cells was determined using Western blotting. ***P < 0.001.