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. 2022 Jan 4;13(1):1411–1423. doi: 10.1080/21655979.2021.2018096

Figure 4.

Figure 4.

CircZNF652 functioned as a sponger by targeting miR-486-5p in GBM cells. (a) The predicated binding sites between circZNF652 and miR-486-5p were speculated using StarBase. (b) The luciferase activity of circZNF652-wt/mut co-transferred with NC mimic or miR-486-5p mimic into 293 T cells. (c) and (d) The mRNA expression of circZNF652 and miR-486-5p in cell lysates with IgG or Ago2 antibodies was examined using RIP assay. (e) The expression of miR-486-5p in A712 and U87 cells treated with NC inhibitor or miR-486-5p inhibitor. (f) and (g) Cell viability was examined using CCK-8 assay after A172 and U87 cells were transferred with si-NC, si-circZNF652, si-circZNF652+ NC inhibitor, si-circZNF652+ miR-486-5p inhibitor. (h) and (k) The colony formation number was measured using colony formation assay after A172 and U87 cells were transferred with si-NC, si-circZNF652, si-circZNF652+ NC inhibitor, si-circZNF652+ miR-486-5p inhibitor. (i)-(j) and (l)-(m) Cell migration and invasion were determined using wound healing assay and transwell assay after A172 and U87 cells were transferred with si-NC, si-circZNF652, si-circZNF652+ NC inhibitor, si-circZNF652+ miR-486-5p inhibitor. (n)-(q) The protein level of E-cadherin, N-cadherin, vimentin was detected using Western blotting after A172 and U87 cells were transferred with si-NC, si-circZNF652, si-circZNF652+ NC inhibitor, si-circZNF652+ miR-486-5p inhibitor. ***P < 0.001.