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. 2022 Jan 4;13(1):1411–1423. doi: 10.1080/21655979.2021.2018096

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Upregulation of circZNF652 reversed the inhibitory effects of miR-486-5p on GBM cell growth, migration, invasion, and EMT process. (a) and (b) Cell viability was examined using CCK-8 assay after A172 and U87 cells were transferred with NC mimic, miR-486-5p mimic, miR-486-5p mimic+pcDNA3.1, miR-486-5p mimic+pcDNA-SERPINE1. (c) and (d) The colony formation number was measured using colony formation assay after A172 and U87 cells were transferred with NC mimic, miR-486-5p mimic, miR-486-5p mimic+pcDNA3.1, miR-486-5p mimic+pcDNA-SERPINE1. (e)-(h) Cell migration and invasion were determined using wound healing assay and transwell assay after A172 and U87 cells were transferred with NC mimic, miR-486-5p mimic, miR-486-5p mimic+pcDNA3.1, miR-486-5p mimic+pcDNA-SERPINE1. (i)-(l) The protein level of E-cadherin, N-cadherin, vimentin was detected using Western blotting after A172 and U87 cells were transferred with NC mimic, miR-486-5p mimic, miR-486-5p mimic+pcDNA3.1, miR-486-5p mimic+pcDNA-SERPINE1. **P < 0.01, ***P < 0.001.