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. 2021 Nov;32(11):2759–2776. doi: 10.1681/ASN.2021040460

Figure 7.

Figure 7.

The expression of GPX4 was regulated by ATF3 in Pkd1 mutant renal epithelial cells. (A) qRT-PCR and Western blot analysis of ATF3 and GPX4 mRNA levels in PH2 and PN24 cells. n=3 independent experiments. (B) qRT-PCR and Western blot analysis of ATF3 and GPX4 mRNA levels in Pkd1 WT and Pkd1 null MEK cells. n=3 independent experiments. (C) qRT-PCR and Western blot analysis of the expression of ATF3 in kidneys from 3-month-old WT (n=5) and Pkd1RC/RC mice (n=5). (D) qRT-PCR and Western blot analysis of the expression of ATF3 in kidneys from Pkd1RC/RC mice treated with vehicle (n=5) or erastin (n=5). (E) qRT-PCR and Western blot analysis of the expression of ATF3 in kidneys from Pkd1flox/flox:Pkhd1-Cre mice treated with vehicle (n=5) or Fer-1 (n=5). (F) qRT-PCR and Western blot analysis of the expression of ATF3 and GPX4 in PN24 cells transfected with ATF3 siRNA and control siRNA. n=3 independent experiments. (G and H) chromatin immunoprecipitation (ChIP) (G) and ChIP-qPCR (H) analysis indicated that ATF3 bound to the promoter of GPX4 in PN24 cells. Histone H3 was used as a positive control. Normal rabbit IgG was used as a negative control. Statistical data are presented as the mean±SEM.