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Fig. 2.

Fig. 2

Relative (A) CD34 and (B) SUZ12 mRNA expression levels of CD34+ hematopoietic stem cells in the presence of 0.2 and 0.4 mM LC for 48 h of incubation. CD34+ HSCs were cultured for 48 h in the presence of 0.2 and 0.4 mM LC. Total RNA was then extracted from cultured CD34+ cells in group I as control group (CD34+ HSCs without any LC treatment) and group II and III as experimental groups (CD34+ HSCs with 0.2 and 0.4 mM LC treatment) as described in the methods section and subjected to real-time PCR assay, mean±SEM, n=3, *P<0.05, and **P<0.01. The Y-axis shows the fold of the mRNA expression level