| Genetic reagent (Mus musculus) |
Pomc -TdDsRed
|
PMID:19864580
|
|
Dr Malcolm Low (University of Michigan) |
| Genetic reagent (Mus musculus) |
Pomc-CreERT2
|
PMID:24177424
|
RRID:MGI:5569339
|
|
| Genetic reagent (Mus musculus) |
Rosa26eGFP-L10a
|
Jackson Laboratory |
Stock # 024750;RRID: IMSR_JAX:024750
|
|
| Antibody |
Anti-Nr5a1(Rabbit Polyclonal) |
Dr Gary Hammer, University of Michigan |
|
IF (1:750–1:1000) |
| Antibody |
Anti-td-Tomato [16D7] (Rat Monoclonal) |
Kerafast |
Cat # EST203;RRID:AB_2732803
|
IF (1:750) |
| Antibody |
Anti-Pomc (Rabbit Polyclonal) |
Phoenix Pharmaceuticals |
Cat # H-029–30;RRID:AB_2307442
|
IF(1:1000) |
| Antibody |
Anti-Rabbit Alexa 488 (Goat polyclonal) |
Invitrogen |
Cat # A-11034;RRID:AB_2576217
|
IF(1:500–1:1000) |
| Antibody |
Anti-Rat Alexa Fluor 555 (Goat polyclonal) |
Invitrogen |
Cat # A-21434;RRID:AB_2535855
|
IF(1:500–1:1000) |
| Antibody |
Anti-Esr1 (Rabbit Polyclonal) |
Dr Sue Moenter, University of Michigan |
|
IF(1:10000) |
| Antibody |
Anti-GFP (Chicken polyclonal) |
Abcam |
Cat # ab13970;RRID:AB_300798
|
IF(1:1000) |
| Chemical compound, drug |
Papain Dissociation System |
Worthington Biochemical Corporation |
LK003178 |
|
| Chemical compound, drug |
DNase |
Worthington Biochemical Corporation |
LK003172 |
|
| Software, algorithm |
R |
R Project for Statistical Computing |
https://www.r-project.org/
|
|
| Software, algorithm |
Seurat 3.1.5 |
Satija Lab |
https://satijalab.org/seurat/articles/install.html
|
|
| Software, algorithm |
Monocle 2 |
Cole-trapnell lab |
http://cole-trapnell-lab.github.io/monocle-release/docs/
|
|
| Software, algorithm |
Destiny 2.14.0 |
Carsten Marr & Florian Buettner lab |
https://theislab.github.io/destiny/
|
|
| Sequence-based reagent |
RNAscope Multiplex Fluorescent V2 Assay |
ACDbio |
Cat # 323110 |
|
| Sequence-based reagent |
Mm-Six3
|
Advanced Cell Diagnostics |
Cat # 412941-C3 |
|
| Sequence-based reagent |
Mm-Six6
|
Advanced Cell Diagnostics |
Cat # 574291 |
|
| Sequence-based reagent |
Mm-Tac2
|
Advanced Cell Diagnostics |
Cat # 446391-C3 |
|
| Sequence-based reagent |
Mm-Npy
|
Advanced Cell Diagnostics |
Cat # 313321 |
|
| Sequence-based reagent |
Mm-Ebf1
|
Advanced Cell Diagnostics |
Cat # 433411 |
|
| Sequence-based reagent |
Mm-Pou4f1
|
Advanced Cell Diagnostics |
Cat # 414671-C3 |
|
| Sequence-based reagent |
Mm-Pomc
|
Advanced Cell Diagnostics |
Cat # 314081-C2 |
|
| Sequence-based reagent |
Mm-Sox14
|
Advanced Cell Diagnostics |
Cat # 516411 |
|
| Sequence-based reagent |
Mm-Prdm13
|
Advanced Cell Diagnostics |
Cat # 543551-C2 |
|
| Sequence-based reagent |
Mm-Tacr3
|
Advanced Cell Diagnostics |
Cat # 481671 |
|
| Sequence-based reagent |
Mm-Dlx5
|
Advanced Cell Diagnostics |
Cat # 478151 |
|
| Sequence-based reagent |
Mm-Pitx2
|
Advanced Cell Diagnostics |
Cat # 412841 |
|
| Sequence-based reagent |
Mm-Pomc
|
Advanced Cell Diagnostics |
Cat # 314081 |
|
| Sequence-based reagent |
Mm-Positive Control Probe |
Advanced Cell Diagnostics |
Cat # 320881 |
|
| Sequence-based reagent |
Mm-Negative Control Probe |
Advanced Cell Diagnostics |
Cat # 320871 |
|
| Sequence-based reagent |
RNAscope Probe Diluent |
Advanced Cell Diagnostics |
Cat # 300041 |
|
| Chemical compound, drug |
Opal 520 Reagent Pack |
AKOYA Biosciences |
Cat # NC1601877 |
1:750–1:1500 |
| Chemical compound, drug |
Opal 620 Reagent Pack |
AKOYA Biosciences |
Cat # NC1612059 |
1:750–1:1500 |
| Chemical compound, drug |
Opal 690 Reagent Pack |
AKOYA Biosciences |
Cat # NC1605064 |
1:750–1:1500 |
| Other |
DAPI |
BD Biosciences |
Cat # 564907 |
FACS (500–1000 ng/mL) |
| Other |
ProLong Gold Antifade Mountant |
ThermoFisher |
P36930 |
|
| Other |
Earle’s Balanced Salts |
Sigma-Aldrich |
E2888 |
|
