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. 2022 Jan 10;11:e74589. doi: 10.7554/eLife.74589

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Chemical compound, drug 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'rac-glycerol) (18:1 06:0 POPG) Avanti Polar Lipids 840457 C  
Chemical compound, drug E. coli polar extract Avanti Polar Lipids 100600 P  
Chemical compound, drug 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (18:1 06:0 POPE) Avanti Polar Lipids 850757 C  
Chemical compound, drug Triton X-100 Sigma Cat#T9284  
Chemical compound, drug Lysozyme Applichem Cat#A3711  
Chemical compound, drug Benzamidine Sigma B6506  
Chemical compound, drug Chloroform Fluka 25,690  
Chemical compound, drug DM Anatrace D322  
Chemical compound, drug DDM Anatrace D310S  
Chemical compound, drug Diethyl ether Sigma 296,082  
Chemical compound, drug DNase I AppliChem A3778  
Chemical compound, drug glycerol 99% Sigma G7757  
Chemical compound, drug HCl Merck Millipore 1.00319.1000  
Chemical compound, drug HEPES Sigma H3375  
Chemical compound, drug Imidazole Roth X998.4  
Chemical compound, drug L-(+)-arabinose Sigma A3256  
Chemical compound, drug Leupeptin AppliChem A2183  
Chemical compound, drug Pepstatin AppliChem A2205  
Chemical compound, drug Valinomycin Thermofischer Scientific V1644  
Chemical compound, drug Calcimycin Thermofischer Scientific A1493  
Chemical compound, drug Ionomycin Thermofischer Scientific I24222  
Chemical compound, drug Fura2 Thermofischer Scientific F1200  
Chemical compound, drug Magnesium Green Thermofischer Scientific M3733  
Chemical compound, drug ACMA Thermofischer Scientific A1324  
Chemical compound, drug CCCP Sigma C2759  
Chemical compound, drug Calcein Thermofischer Scientific C481  
Chemical compound, drug Phosphate buffered saline Sigma D8537  
Chemical compound, drug PEG400 Sigma 91,893  
Chemical compound, drug Manganese acetate Sigma 330,825  
Chemical compound, drug Magnesium acetate Fluka 63,047  
Chemical compound, drug Calcium Chloride Sigma 223,506  
Chemical compound, drug Manganese Chloride Fluka 31,422  
Chemical compound, drug Magnesium Chloride Fluka 63,065  
Chemical compound, drug PMSF Sigma P7626  
Chemical compound, drug Potassium chloride Sigma 746,346  
Chemical compound, drug Sodium chloride Sigma 71,380  
Chemical compound, drug Terrific broth Sigma T9179  
Commercial assay or kit 4%–20% Mini-PROTEAN TGX Precast Protein Gels, 15-well, 15 µl BioRad Laboratories 4561096DC  
Commercial assay or kit Amicon Ultra-4 Centrifugal Filters Ultracel 10 K, 4 ml Merck Millipore UFC801096  
Commercial assay or kit Amicon Ultra-4 Centrifugal Filters Ultracel 50 K, 4 ml Merck Millipore UFC805096  
Commercial assay or kit Amicon Ultra-4 Centrifugal Filters Ultracel 100 K, 4 ml Merck Millipore UFC810024  
Commercial assay or kit Biobeads SM-2 adsorbents BioRad Laboratories 152–3920  
Commercial assay or kit Avestin Extruder kit Sigma Cat#Z373400  
Commercial assay or kit 400 nm polycarbonate filters Sigma Cat#Z373435  
Commercial assay or kit 96-well black walled microplates Thermofischer Scientific Cat#M33089  
Commercial assay or kit Ni-NTA resin ABT Agarose Bead Technologies 6BCL-NTANi-X  
Commercial assay or kit QuantiFoil R1.2/1.3 Au 200 mesh Electron Microscopy Sciences Q2100AR1.3  
Commercial assay or kit Superdex 200 10/300 GL Cytiva 17517501  
Commercial assay or kit Superdex 200 Increase 3.2/300 Cytiva 28990946  
Commercial assay or kit Superdex 200 Increase 5/150 GL Cytiva 28990945  
Commercial assay or kit Superdex 75 10/300 GL Cytiva 17517401  
Other BioQuantum Energy Filter Gatan NA  
Other HPL6 Maximator NA  
Other K3 Direct Detector Gatan NA  
Other Titan Krios G3i ThermoFisher Scientific NA  
Other Viber Fusion FX7 imaging system Witec NA  
Other TECAN M1000 Infinite TECAN NA  
Other Vitrobot Mark IV ThermoFisher Scientific NA  
Other μDAWN MALS Detector Wyatt Technology NA  
Recombinant DNA reagent gDNA Eggerthella lenta DSMZ 2,243  
Recombinant DNA reagent gDNA Eremococcus coleocola DSMZ 15,696  
Recombinant DNA reagent Bacterial expression vector with C-terminal 3 C cleavage site, GFP-tag and His-tag Dutzler laboratory NA  
Recombinant DNA reagent Bacterial expression vector with N-terminal His-tag and 3 C cleavage site Dutzler laboratory NA  
Recombinant DNA reagent Bacterial expression vector with N terminal pelB sequence, His-tag, MBP, 3 C cleavage site Dutzler laboratory NA  
Recombinant protein HRV 3 C protease Expressed (pET_3 C) and purified in Dutzler laboratory NA  
Software, algorithm 3DFSC Tan et al., 2017 https://3dfsc.salk.edu/  
Software, algorithm ASTRA7.2 Wyatt Technology https://www.wyatt.com/products/software/astra.html RRID:SCR_016255
Software, algorithm Chimera v.1.15 Pettersen et al., 2004 https://www.cgl.ucsf.edu/chimera/ RRID:SCR_004097
Software, algorithm ChimeraX v.1.1.1 Pettersen et al., 2021 https://www.rbvi.ucsf.edu/chimerax/ RRID:SCR_015872
Software, algorithm Coot v.0.9.4 Emsley et al., 2010 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ RRID:SCR_014222
Software, algorithm cryoSPARC v.3.0.1/v.3.2.0 Structural Biotechnology Inc. https://cryosparc.com/ RRID:SCR_016501
Software, algorithm DINO   http://www.dino3d.org RRID:SCR_013497
Software, algorithm EPU2.9 ThermoFisher Scientific NA  
Software, algorithm Phenix Liebschner et al., 2019 https://www.phenix-online.org/ RRID:SCR_014224
Software, algorithm RELION 3.0.7 Zivanov et al., 2018 https://www3.mrc-lmb.cam.ac.uk/relion/ RRID:SCR_016274
Software, algorithm JALVIEW Waterhouse et al., 2009  
Software, algorithm Muscle Edgar, 2004  
Strain, strain background (E coli) E. coli MC1061 ThermoFisher Scientific C66303