Author response image 1. Figure 1.

(A) Cells were transfected with the indicated siRNAs and fixed 48 hours later. mRNAs, DNA and membranes were labeled with smFISH probes, DAPI and fluorescent wheat germ agglutinin, respectively. mRNA numbers of cells in late mitosis were counted and divided by the number of mRNAs present in cells in early mitosis. (B) Cells were transfected with the indicated siRNAs. 48 hours later cells were lysed and the RNA was harvested. Gene-expression levels were analyzed by qPCR, relative to cells treated with siRNAs targeting Luciferase.