Author response image 2. Comparison of immediate decay versus delayed decay in single cells.

We generated two metagenes, one including all genes belonging to the ‘immediate decay’ wave, and one including all genes belonging to the ‘delayed decay’ wave. Metagenes were created by summing up all reads for the genes that belonging to the same wave of decay in a single cell, thus creating one expression value per metagene per cell. Then we analyzed metagene expression at 240 minutes post metaphase normalized to its expression at G2/M.