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. 2021 Mar 4;12(1):763–778. doi: 10.1080/21655979.2021.1880717

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Overexpression of TGF-β1 rescued the effect of NK cells and IL-18

a. Overexpression of TGF-β1 in HCT116 cells was confirmed via qPCR. b. qPCR results indicate that the expression of miR-574-3p was unperturbed when TGF-β1 was overexpressed compared with HCT116 cells tranfected with LV003 vector. c. MTS assays showing that TGF-β1 overexpression restored the proliferative promotional effect on HCT116 cells from miR-574-3p overexpression with NK cells coculture and IL-18 treatment. d. Representative fluorescence-activated cells sorting of 7-AAD-positive and annexin V-positive cells when TGF-β1 is overexpressed. TGF-β1 restored the inhibited apoptotic effect of HCT116 from miR-574-3p overexpression under NK cells and IL-18 treatment. NK: Natural killer; IL-18: Interleukin 18; MTS: 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium; qPCR: Quantitative polymerase chain reaction; TGF-β1: transforming growth factor beta 1. All data with error bars are presented as mean + SD, One-way analysis: * p < 0.05; ns: not significant.