Table 1.
Primers used in qRT-PCR
| Primer | Sequence (5ʹ-3ʹ) | |
|---|---|---|
| GAPDH | Forward | 5ʹ-GACAGTCAGCCGCATCTTCT-3’ |
| Reverse | 5ʹ-GCGCCCAATACGACCAAATC-3’ | |
| U6 | Forward | 5ʹ-CTCGCTTCGGCAGCACA-3’ |
| Reverse | 5ʹ-AACGCTTCACGAATTTGCGT-3’ | |
| lncRNA XIST | Forward | 5ʹ-CCCTCATCCCCACTTTTCCC-3’ |
| Reverse | 5ʹ-TGGAATGAGCAGTGTGCGAT-3’ | |
| miR-4775 | Forward | 5ʹ-TTAATTTTTTGTTTCGG-3’ |
| Reverse | 5ʹ-CAGTGCGTGTCGTGGAGT-3’ | |
| miR-424-3p | Forward | 5ʹ-CAGCAGCAATTCATGTT-3’ |
| Reverse | 5ʹ-CAGTGCGTGTCGTGGAGT-3’ | |
| PLA2G7 | Forward | 5ʹ-CGCAGGGCATTGCCAACTAT-3’ |
| Reverse | 5ʹ-TCCTTTCCGCAGACCTGATG-3’ | |
| AMOT | Forward | 5ʹ-TTTGCCTTCAGGGAGCTGCTA-3’ |
| Reverse | 5ʹ-GTCCAACCACTGGTCACTCT-3’ | |
| TGFBR3 | Forward | 5ʹ-TTTCCTCTTCCCAGCGAGTG-3’ |
| Reverse | 5ʹ-TGCAATTTTCAAACTGCCTCT-3’ | |
qRT-PCR, quantitative real-time polymerase chain reaction; lncRNA, long noncoding RNA; miR, microRNA.