Figure 3.

BTG2 is a miR-92a-3p target (a) The binding sequence of miR-92a-3p with BTG2 was predicted through Bioinformatics analysis. (b) Pearson correlation analysis was conducted to analyze the correlation between miR-92a-3p expression and BTG2 mRNA expression in 60 BC tissues. (c) Dual-luciferase reporter gene assay was utilized for detecting the luciferase activity of BTG2 3ʹUTR WT or MUT after miR-92a-3p was overexpressed. D and E. Western blot and qRT-PCR were carried out to examine BTG2 mRNA and protein expressions in BT549 and MCF-7 cells with transfection of 50 nM miR-92a-3p mimics (or 50 nM miR NC) and 50 nM miR-92a-3p inhibitors (or 50 nM miR in)

The experiments were performed in triplicate. **P< 0.01 and ***P< 0.001, NS, P> 0.05 vs miR NC or miR in group.